Welcome to the Eicosanoid Core Laboratory! 

Eicosanoid is an umbrella term used to describe any oxidized derivative of arachidonic acid, a ubiquitous polyunsaturated fatty acid (PUFA) in humans. Arachidonic acid can be oxidized in vivo via both enzymatic and non-enzymatic mechanisms to yield bioactive lipid mediators that are of central importance to human physiology and pathophysiology.  

The Eicosanoid Core Laboratory at Vanderbilt University was established in 1988 in order to provide investigators at the university with a central location for the analysis of oxidized fatty acids and their metabolites including isoprostanes, prostaglandins, leukotrienes, hydroxy-PUFAs (HETEs), and epoxy-PUFAs (EETs). Today the Eicosanoid Core Laboratory serves investigators not only at Vanderbilt but indeed around the world.  Click here for available assays.

The Eicosanoid Core Lab provides services in three general areas:

Here you can find the Eicosanoid Core's Shipping Policy and Publication Policy.

From right to left: (Back Row) Dawei S. Wei, Stephanie Sanchez, Holly Borntrager(Front Row) Ginger Milne, Warda Amin, Benlian Gao, and Sarah Thomas 

 

Publications

Featured publications

  1. Urinary metabolites of prostanoids and risk of recurrent colorectal adenomas in the Aspirin/Folate Polyp Prevention Study (AFPPS). Fedirko V, Bradshaw PT, Figueiredo JC, Sandler RS, Barry EL, Ahnen DJ, Milne GL, Bresalier RS, Baron JA (2015) Cancer Prev Res (Phila) 8(11): 1061-8
    › Primary publication · 26304466 (PubMed) · PMC4633350 (PubMed Central)
  2. TISSUE REGENERATION. Inhibition of the prostaglandin-degrading enzyme 15-PGDH potentiates tissue regeneration. Zhang Y, Desai A, Yang SY, Bae KB, Antczak MI, Fink SP, Tiwari S, Willis JE, Williams NS, Dawson DM, Wald D, Chen WD, Wang Z, Kasturi L, Larusch GA, He L, Cominelli F, Di Martino L, Djuric Z, Milne GL, Chance M, Sanabria J, Dealwis C, Mikkola D, Naidoo J, Wei S, Tai HH, Gerson SL, Ready JM, Posner B, Willson JK, Markowitz SD (2015) Science 348(6240): aaa2340
    › Primary publication · 26068857 (PubMed) · PMC4481126 (PubMed Central)
  3. Quantification of major urinary metabolites of PGE2 and PGD2 in cystic fibrosis: correlation with disease severity. Jabr S, Gartner S, Milne GL, Roca-Ferrer J, Casas J, Moreno A, Gelpí E, Picado C (2013) Prostaglandins Leukot Essent Fatty Acids 89(2-3): 121-6
    › Primary publication · 23791427 (PubMed)
  4. Effect of zileuton and celecoxib on urinary LTE4 and PGE-M levels in smokers. Mohebati A, Milne GL, Zhou XK, Duffield-Lillico AJ, Boyle JO, Knutson A, Bosworth BP, Kingsley PJ, Marnett LJ, Brown PH, Akpa EG, Szabo E, Dannenberg AJ (2013) Cancer Prev Res (Phila) 6(7): 646-55
    › Primary publication · 23682075 (PubMed) · PMC3707304 (PubMed Central)
  5. Differential stem- and progenitor-cell trafficking by prostaglandin E2. Hoggatt J, Mohammad KS, Singh P, Hoggatt AF, Chitteti BR, Speth JM, Hu P, Poteat BA, Stilger KN, Ferraro F, Silberstein L, Wong FK, Farag SS, Czader M, Milne GL, Breyer RM, Serezani CH, Scadden DT, Guise TA, Srour EF, Pelus LM (2013) Nature 495(7441): 365-9
    › Primary publication · 23485965 (PubMed) · PMC3606692 (PubMed Central)
  6. Cyclooxygenase-1, not cyclooxygenase-2, is responsible for physiological production of prostacyclin in the cardiovascular system. Kirkby NS, Lundberg MH, Harrington LS, Leadbeater PD, Milne GL, Potter CM, Al-Yamani M, Adeyemi O, Warner TD, Mitchell JA (2012) Proc Natl Acad Sci U S A 109(43): 17597-602
    › Primary publication · 23045674 (PubMed) · PMC3491520 (PubMed Central)
  7. Measurement of F2- isoprostanes and isofurans using gas chromatography-mass spectrometry. Milne GL, Gao B, Terry ES, Zackert WE, Sanchez SC (2013) Free Radic Biol Med : 36-44
    › Primary publication · 23044261 (PubMed) · PMC4306329 (PubMed Central)
  8. Major metabolite of F2-isoprostane in urine may be a more sensitive biomarker of oxidative stress than isoprostane itself. Dorjgochoo T, Gao YT, Chow WH, Shu XO, Yang G, Cai Q, Rothman N, Cai H, Li H, Deng X, Franke A, Roberts LJ, Milne G, Zheng W, Dai Q (2012) Am J Clin Nutr 96(2): 405-14
    › Primary publication · 22760572 (PubMed) · PMC3396448 (PubMed Central)
  9. Isoprostane generation and function. Milne GL, Yin H, Hardy KD, Davies SS, Roberts LJ (2011) Chem Rev 111(10): 5973-96
    › Primary publication · 21848345 (PubMed) · PMC3192249 (PubMed Central)
  10. Nonenzymatic free radical-catalyzed generation of 15-deoxy-Δ(12,14)-prostaglandin J₂-like compounds (deoxy-J₂-isoprostanes) in vivo. Hardy KD, Cox BE, Milne GL, Yin H, Roberts LJ (2011) J Lipid Res 52(1): 113-24
    › Primary publication · 20944061 (PubMed) · PMC2999919 (PubMed Central)

The Eicosanoid Core is supported by NIH grant DK020593.  Please acknowledge this in your publications.

Community Leaders

Contact Information

23rd Ave South at Pierce Ave
561 Preston Research Bldg
Nashville, TN 37232-6602
United States
615-936-2050 (p)

Ginger Milne
936-5611 (p)
Email

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

20-HETE Amniotic Fluid Cerebrovascular Circulation Chi-Square Distribution Diet, High-Fat Drug Administration Schedule Dyslipidemias EETs Eicosanoid F2-Isoprostanes Fibroblasts Hematopoietic Stem Cell Mobilization Integrases Intestinal Diseases Iron Isoprostane Leukotriene Mass spectrometry Matrix Metalloproteinase 9 Maximum Tolerated Dose Models, Biological Oxidative Stress Prospective Studies Prostaglandin-E Synthases prostaglandin biosynthesis Pyrazoles Quercetin