Our multi-disciplinary research program involves the development, validation, and translation of non-invasive molecular imaging metrics to assess disease progression and response to therapy. Projects within our program emphasize molecular imaging of cancer, and notably imaging-based assessment of response to anti-cancer pharmaceuticals in colorectal and breast cancer. We are actively engaged in advancing novel methods to image molecular events in both humans and animal models that include receptor expression, proliferation, angiogenesis, and apoptosis. Among these methods include validation of imaging probes for novel applications, as well as high-throughput small-molecule discovery to develop novel imaging compounds.


The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Evaluation of the novel TSPO radiotracer 2-(7-butyl-2-(4-(2-([F]fluoroethoxy)phenyl)-5-methylpyrazolo[1,5-a]pyrimidin-3-yl)-N,N-diethylacetamide in a preclinical model of neuroinflammation. Tang D, Fujinaga M, Hatori A, Zhang Y, Yamasaki T, Xie L, Mori W, Kumata K, Liu J, Manning HC, Huang G, Zhang MR (2018) Eur J Med Chem : 1-8
    › Primary publication · 29505933 (PubMed)
  2. DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer. Luo N, Nixon MJ, Gonzalez-Ericsson PI, Sanchez V, Opalenik SR, Li H, Zahnow CA, Nickels ML, Liu F, Tantawy MN, Sanders ME, Manning HC, Balko JM (2018) Nat Commun 9(1): 248
    › Primary publication · 29339738 (PubMed) · PMC5770411 (PubMed Central)
  3. Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models. Schulte ML, Fu A, Zhao P, Li J, Geng L, Smith ST, Kondo J, Coffey RJ, Johnson MO, Rathmell JC, Sharick JT, Skala MC, Smith JA, Berlin J, Washington MK, Nickels ML, Manning HC (2018) Nat Med 24(2): 194-202
    › Primary publication · 29334372 (PubMed) · PMC5803339 (PubMed Central)
  4. Intracardiac Metastases Detected by 18F-FSPG PET/CT. Magarik MA, Walker RC, Gilbert J, Manning HC, Massion PP (2018) Clin Nucl Med 43(1): 28-30
    › Primary publication · 29076915 (PubMed) · PMC5716866 (PubMed Central)
  5. Optimized Translocator Protein Ligand for Optical Molecular Imaging and Screening. Li J, Smith JA, Dawson ES, Fu A, Nickels ML, Schulte ML, Manning HC (2017) Bioconjug Chem 28(4): 1016-1023
    › Primary publication · 28156095 (PubMed)
  6. F-Fluoroestradiol PET/CT Measurement of Estrogen Receptor Suppression during a Phase I Trial of the Novel Estrogen Receptor-Targeted Therapeutic GDC-0810: Using an Imaging Biomarker to Guide Drug Dosage in Subsequent Trials. Wang Y, Ayres KL, Goldman DA, Dickler MN, Bardia A, Mayer IA, Winer E, Fredrickson J, Arteaga CL, Baselga J, Manning HC, Mahmood U, Ulaner GA (2017) Clin Cancer Res 23(12): 3053-3060
    › Primary publication · 28011460 (PubMed) · PMC5474190 (PubMed Central)
  7. A comparative assessment of preclinical chemotherapeutic response of tumors using quantitative non-Gaussian diffusion MRI. Xu J, Li K, Smith RA, Waterton JC, Zhao P, Ding Z, Does MD, Manning HC, Gore JC (2017) Magn Reson Imaging : 195-202
    › Primary publication · 27919785 (PubMed) · PMC5316364 (PubMed Central)
  8. Evaluation of TSPO PET Ligands [F]VUIIS1009A and [F]VUIIS1009B: Tracers for Cancer Imaging. Tang D, Li J, Buck JR, Tantawy MN, Xia Y, Harp JM, Nickels ML, Meiler J, Manning HC (2017) Mol Imaging Biol 19(4): 578-588
    › Primary publication · 27853987 (PubMed) · PMC5634614 (PubMed Central)
  9. Non-Invasive Glutamine PET Reflects Pharmacological Inhibition of BRAF In Vivo. Schulte ML, Hight MR, Ayers GD, Liu Q, Shyr Y, Washington MK, Manning HC (2017) Mol Imaging Biol 19(3): 421-428
    › Primary publication · 27770401 (PubMed) · PMC6476310 (PubMed Central)
  10. Utility of [F]FSPG PET to Image Hepatocellular Carcinoma: First Clinical Evaluation in a US Population. Kavanaugh G, Williams J, Morris AS, Nickels ML, Walker R, Koglin N, Stephens AW, Washington MK, Geevarghese SK, Liu Q, Ayers D, Shyr Y, Manning HC (2016) Mol Imaging Biol 18(6): 924-934
    › Primary publication · 27677886 (PubMed) · PMC5641676 (PubMed Central)
  11. F-FLT Positron Emission Tomography (PET) is a Pharmacodynamic Marker for EWS-FLI1 Activity and Ewing Sarcoma. Osgood CL, Tantawy MN, Maloney N, Madaj ZB, Peck A, Boguslawski E, Jess J, Buck J, Winn ME, Manning HC, Grohar PJ (2016) Sci Rep : 33926
    › Primary publication · 27671553 (PubMed) · PMC5037393 (PubMed Central)
  12. Activation of GRP/GRP-R signaling contributes to castration-resistant prostate cancer progression. Qiao J, Grabowska MM, Forestier-Roman IS, Mirosevich J, Case TC, Chung DH, Cates JM, Matusik RJ, Manning HC, Jin R (2016) Oncotarget 7(38): 61955-61969
    › Primary publication · 27542219 (PubMed) · PMC5308703 (PubMed Central)
  13. The selective estrogen receptor downregulator GDC-0810 is efficacious in diverse models of ER+ breast cancer. Joseph JD, Darimont B, Zhou W, Arrazate A, Young A, Ingalla E, Walter K, Blake RA, Nonomiya J, Guan Z, Kategaya L, Govek SP, Lai AG, Kahraman M, Brigham D, Sensintaffar J, Lu N, Shao G, Qian J, Grillot K, Moon M, Prudente R, Bischoff E, Lee KJ, Bonnefous C, Douglas KL, Julien JD, Nagasawa JY, Aparicio A, Kaufman J, Haley B, Giltnane JM, Wertz IE, Lackner MR, Nannini MA, Sampath D, Schwarz L, Manning HC, Tantawy MN, Arteaga CL, Heyman RA, Rix PJ, Friedman L, Smith ND, Metcalfe C, Hager JH (2016) Elife
    › Primary publication · 27410477 (PubMed) · PMC4961458 (PubMed Central)
  14. New structure-activity relationships of N-acetamide substituted pyrazolopyrimidines as pharmacological ligands of TSPO. Li J, Schulte ML, Nickels ML, Manning HC (2016) Bioorg Med Chem Lett 26(15): 3472-7
    › Primary publication · 27353534 (PubMed) · PMC4993599 (PubMed Central)
  15. Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting. Higginbotham JN, Zhang Q, Jeppesen DK, Scott AM, Manning HC, Ochieng J, Franklin JL, Coffey RJ (2016) J Extracell Vesicles : 29254
    › Primary publication · 27345057 (PubMed) · PMC4921784 (PubMed Central)
  16. Mouse Models of Breast Cancer: Platforms for Discovering Precision Imaging Diagnostics and Future Cancer Medicine. Manning HC, Buck JR, Cook RS (2016) J Nucl Med : 60S-8S
    › Primary publication · 26834104 (PubMed) · PMC6231414 (PubMed Central)
  17. 2-Amino-4-bis(aryloxybenzyl)aminobutanoic acids: A novel scaffold for inhibition of ASCT2-mediated glutamine transport. Schulte ML, Khodadadi AB, Cuthbertson ML, Smith JA, Manning HC (2016) Bioorg Med Chem Lett 26(3): 1044-1047
    › Primary publication · 26750251 (PubMed) · PMC4727990 (PubMed Central)
  18. Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study. Buck JR, McKinley ET, Fu A, Abel TW, Thompson RC, Chambless L, Watchmaker JM, Harty JP, Cooper MK, Manning HC (2015) PLoS One 10(10): e0141659
    › Primary publication · 26517124 (PubMed) · PMC4627825 (PubMed Central)
  19. Preclinical Evaluation of 4-[18F]Fluoroglutamine PET to Assess ASCT2 Expression in Lung Cancer. Hassanein M, Hight MR, Buck JR, Tantawy MN, Nickels ML, Hoeksema MD, Harris BK, Boyd K, Massion PP, Manning HC (2016) Mol Imaging Biol 18(1): 18-23
    › Primary publication · 25971659 (PubMed) · PMC4644503 (PubMed Central)
  20. [(18)F]-FLT PET to predict early response to neoadjuvant therapy in KRAS wild-type rectal cancer: a pilot study. McKinley ET, Watchmaker JM, Chakravarthy AB, Meyerhardt JA, Engelman JA, Walker RC, Washington MK, Coffey RJ, Manning HC (2015) Ann Nucl Med 29(6): 535-42
    › Primary publication · 25899481 (PubMed) · PMC4503488 (PubMed Central)
  21. World Molecular Imaging Congress 2015: precision medicine visualized. Manning HC (2015) Mol Imaging Biol 17(3): 295-6
    › Primary publication · 25862478 (PubMed)
  22. High-yielding, automated production of 3'-deoxy-3'-[(18)F]fluorothymidine using a modified Bioscan Coincidence FDG reaction module. Cheung YY, Nickels ML, McKinley ET, Buck JR, Manning HC (2015) Appl Radiat Isot : 47-51
    › Primary publication · 25531913 (PubMed) · PMC4867542 (PubMed Central)
  23. Acetate dependence of tumors. Comerford SA, Huang Z, Du X, Wang Y, Cai L, Witkiewicz AK, Walters H, Tantawy MN, Fu A, Manning HC, Horton JD, Hammer RE, McKnight SL, Tu BP (2014) Cell 159(7): 1591-602
    › Primary publication · 25525877 (PubMed) · PMC4272450 (PubMed Central)
  24. 2-Substituted Nγ-glutamylanilides as novel probes of ASCT2 with improved potency. Schulte ML, Dawson ES, Saleh SA, Cuthbertson ML, Manning HC (2015) Bioorg Med Chem Lett 25(1): 113-6
    › Primary publication · 25435145 (PubMed) · PMC4372298 (PubMed Central)
  25. Molecular imaging of folate receptor β-positive macrophages during acute lung inflammation. Han W, Zaynagetdinov R, Yull FE, Polosukhin VV, Gleaves LA, Tanjore H, Young LR, Peterson TE, Manning HC, Prince LS, Blackwell TS (2015) Am J Respir Cell Mol Biol 53(1): 50-9
    › Primary publication · 25375039 (PubMed) · PMC4566110 (PubMed Central)
  26. 3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer. McKinley ET, Zhao P, Coffey RJ, Washington MK, Manning HC (2014) PLoS One 9(9): e108193
    › Primary publication · 25247710 (PubMed) · PMC4172755 (PubMed Central)
  27. Facile synthesis of SSR180575 and discovery of 7-chloro-N,N,5-trimethyl-4-oxo-3(6-[(18)F]fluoropyridin-2-yl)-3,5-dihydro-4H-pyridazino[4,5-b]indole-1-acetamide, a potent pyridazinoindole ligand for PET imaging of TSPO in cancer. Cheung YY, Nickels ML, Tang D, Buck JR, Manning HC (2014) Bioorg Med Chem Lett 24(18): 4466-4471
    › Primary publication · 25172419 (PubMed) · PMC4163096 (PubMed Central)
  28. Utility of [18 F]FLT-PET to assess treatment response in trastuzumab-resistant and trastuzumab-sensitive HER2-overexpressing human breast cancer xenografts. Whisenant JG, McIntyre JO, Peterson TE, Kang H, Sánchez V, Manning HC, Arteaga CL, Yankeelov TE (2015) Mol Imaging Biol 17(1): 119-28
    › Primary publication · 25034624 (PubMed) · PMC4311727 (PubMed Central)
  29. Preclinical imaging evaluation of novel TSPO-PET ligand 2-(5,7-Diethyl-2-(4-(2-[(18)F]fluoroethoxy)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)-N,N-diethylacetamide ([ (18)F]VUIIS1008) in glioma. Tang D, Nickels ML, Tantawy MN, Buck JR, Manning HC (2014) Mol Imaging Biol 16(6): 813-20
    › Primary publication · 24845529 (PubMed) · PMC4372299 (PubMed Central)
  30. Inducible loss of one Apc allele in Lrig1-expressing progenitor cells results in multiple distal colonic tumors with features of familial adenomatous polyposis. Powell AE, Vlacich G, Zhao ZY, McKinley ET, Washington MK, Manning HC, Coffey RJ (2014) Am J Physiol Gastrointest Liver Physiol 307(1): G16-23
    › Primary publication · 24833705 (PubMed) · PMC4080164 (PubMed Central)
  31. PET radiotracer [¹⁸F]-P6 selectively targeting COX-1 as a novel biomarker in ovarian cancer: preliminary investigation. Perrone MG, Malerba P, Uddin J, Vitale P, Panella A, Crews BC, Daniel CK, Ghebreselasie K, Nickels M, Tantawy MN, Manning HC, Marnett LJ, Scilimati A (2014) Eur J Med Chem : 562-568
    › Primary publication · 24832612 (PubMed) · PMC4401082 (PubMed Central)
  32. A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells. Hight MR, Cheung YY, Nickels ML, Dawson ES, Zhao P, Saleh S, Buck JR, Tang D, Washington MK, Coffey RJ, Manning HC (2014) Clin Cancer Res 20(8): 2126-35
    › Primary publication · 24573549 (PubMed) · PMC3989451 (PubMed Central)
  33. Microwave-assisted, one-pot reaction of 7-azaindoles and aldehydes: a facile route to novel di-7-azaindolylmethanes. Uddin MI, Buck JR, Schulte ML, Tang D, Saleh SA, Cheung YY, Harp J, Manning HC (2014) Tetrahedron Lett 55(1)
    › Primary publication · 24396154 (PubMed) · PMC3880151 (PubMed Central)
  34. Global phosphotyrosine proteomics identifies PKCδ as a marker of responsiveness to Src inhibition in colorectal cancer. McKinley ET, Liu H, McDonald WH, Luo W, Zhao P, Coffey RJ, Hanks SK, Manning HC (2013) PLoS One 8(11): e80207
    › Primary publication · 24260357 (PubMed) · PMC3832668 (PubMed Central)
  35. Translocator protein 18 kDa (TSPO) is regulated in white and brown adipose tissue by obesity. Thompson MM, Manning HC, Ellacott KL (2013) PLoS One 8(11): e79980
    › Primary publication · 24260329 (PubMed) · PMC3832377 (PubMed Central)
  36. A novel reporter system for molecular imaging and high-throughput screening of anticancer drugs. Xie J, Wang C, Virostko J, Manning HC, Pham W, Bauer J, Gore JC (2013) Chembiochem 14(12): 1494-503
    › Primary publication · 23881799 (PubMed) · PMC3819221 (PubMed Central)
  37. Knockdown of aberrantly upregulated aryl hydrocarbon receptor reduces tumor growth and metastasis of MDA-MB-231 human breast cancer cell line. Goode GD, Ballard BR, Manning HC, Freeman ML, Kang Y, Eltom SE (2013) Int J Cancer 133(12): 2769-80
    › Primary publication · 23733406 (PubMed) · PMC3797219 (PubMed Central)
  38. Evaluation of CD44 and CD133 as cancer stem cell markers for colorectal cancer. Wang C, Xie J, Guo J, Manning HC, Gore JC, Guo N (2012) Oncol Rep 28(4): 1301-8
    › Primary publication · 22895640 (PubMed) · PMC3981033 (PubMed Central)