Proper coordination of nuclear and cell division is necessary for the normal development of all eukaryotic organisms and for the maintenance of genomic integrity. The goals of my laboratory are two-fold: 1) to understand the molecular mechanisms regulating cytokinesis and 2) to learn how cytokinesis is coordinated with other events of the cell cycle including chromosome segregation and microtubule cytoskeletal rearrangements. We are asking a) what is the full complement of proteins that comprise the cytokinetic machinery, b) how is the cytokinetic machinery precisely localized between segregating chromosomes, c) what molecular event(s) triggers constriction of the cytokinetic machinery, and d) what ensures the correct timing of cytokinesis with respect to chromosome segregation?
To address these questions expeditiously, we are using yeast as a model organism.
Yeast offers many experimental advantages for the study of cell cycle regulation including facile genetics and a fully sequenced and annotated genome. Using genetics, proteomics, live cell imaging, biochemistry, and structural biology we are unraveling the sequence of events that directs formation and then constriction of the cytokinetic ring. We are also studying protein phosphorylation signaling pathways and ubiquitin ligases that control the spatial precision of cytokinesis, the organization of the microtubule cytoskeleton through the cell cycle, and the timing of cytokinetic events during mitosis.


The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division. Bhattacharjee R, Mangione MC, Wos M, Chen JS, Snider CE, Roberts-Galbraith RH, McDonald NA, Presti LL, Martin SG, Gould KL (2020) Mol Biol Cell 31(9): 917-929
    › Primary publication · 32101481 (PubMed) · PMC7185970 (PubMed Central)
  2. The intrinsically disordered region of the cytokinetic F-BAR protein Cdc15 performs a unique essential function in maintenance of cytokinetic ring integrity. Mangione MC, Snider CE, Gould KL (2019) Mol Biol Cell 30(22): 2790-2801
    › Primary publication · 31509478 (PubMed) · PMC6789166 (PubMed Central)
  3. The adaptor protein Bbc1 regulates localization of Wsp1 and Vrp1 during endocytic actin patch assembly. MacQuarrie CD, Mangione MC, Carroll R, James M, Gould KL, Sirotkin V (2019) J Cell Sci 132(17)
    › Primary publication · 31391237 (PubMed) · PMC6771142 (PubMed Central)
  4. Molecular form and function of the cytokinetic ring. Mangione MC, Gould KL (2019) J Cell Sci 132(12)
    › Primary publication · 31209062 (PubMed) · PMC6602304 (PubMed Central)
  5. NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement. Willet AH, DeWitt AK, Beckley JR, Clifford DM, Gould KL (2019) Curr Biol 29(6): 1055-1063.e2
    › Primary publication · 30853434 (PubMed) · PMC6424596 (PubMed Central)
  6. The F-BAR Domain of Rga7 Relies on a Cooperative Mechanism of Membrane Binding with a Partner Protein during Fission Yeast Cytokinesis. Liu Y, McDonald NA, Naegele SM, Gould KL, Wu JQ (2019) Cell Rep 26(10): 2540-2548.e4
    › Primary publication · 30840879 (PubMed) · PMC6425953 (PubMed Central)
  7. Spatiotemporal regulation of the Dma1-mediated mitotic checkpoint coordinates mitosis with cytokinesis. Cullati SN, Gould KL (2019) Curr Genet 65(3): 663-668
    › Primary publication · 30600396 (PubMed) · PMC6511297 (PubMed Central)
  8. Cdk1-dependent phosphoinhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring formation. Willet AH, Bohnert KA, Gould KL (2018) Mol Biol Cell 29(6): 713-721
    › Primary publication · 29343550 (PubMed) · PMC6003227 (PubMed Central)
  9. Nanoscale architecture of the contractile ring. McDonald NA, Lind AL, Smith SE, Li R, Gould KL (2017) Elife
    › Primary publication · 28914606 (PubMed) · PMC5779233 (PubMed Central)
  10. Providing Experiential Business and Management Training for Biomedical Research Trainees. Petrie KA, Carnahan RH, Brown AM, Gould KL (2017) CBE Life Sci Educ 16(3)
    › Primary publication · 28798213 (PubMed) · PMC5589431 (PubMed Central)
  11. Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis. Snider CE, Willet AH, Chen JS, Arpağ G, Zanic M, Gould KL (2017) J Cell Biol 216(10): 3041-3050
    › Primary publication · 28784611 (PubMed) · PMC5626552 (PubMed Central)
  12. The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis. McDonald NA, Takizawa Y, Feoktistova A, Xu P, Ohi MD, Vander Kooi CW, Gould KL (2016) Cell Rep 14(3): 534-546
    › Primary publication · 26776521 (PubMed) · PMC4731314 (PubMed Central)
  13. Temporal Regulation of Lipin Activity Diverged to Account for Differences in Mitotic Programs. Makarova M, Gu Y, Chen JS, Beckley JR, Gould KL, Oliferenko S (2016) Curr Biol 26(2): 237-243
    › Primary publication · 26774782 (PubMed) · PMC4728079 (PubMed Central)
  14. Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis. McDonald NA, Vander Kooi CW, Ohi MD, Gould KL (2015) Dev Cell 35(6): 725-36
    › Primary publication · 26702831 (PubMed) · PMC4691284 (PubMed Central)
  15. The DYRK-family kinase Pom1 phosphorylates the F-BAR protein Cdc15 to prevent division at cell poles. Ullal P, McDonald NA, Chen JS, Lo Presti L, Roberts-Galbraith RH, Gould KL, Martin SG (2015) J Cell Biol 211(3): 653-68
    › Primary publication · 26553932 (PubMed) · PMC4639868 (PubMed Central)
  16. Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins. McDonald NA, Gould KL (2016) Methods Mol Biol : 181-9
    › Primary publication · 26519313 (PubMed)
  17. The origin and implementation of the Broadening Experiences in Scientific Training programs: an NIH common fund initiative. Meyers FJ, Mathur A, Fuhrmann CN, O'Brien TC, Wefes I, Labosky PA, Duncan DS, August A, Feig A, Gould KL, Friedlander MJ, Schaffer CB, Van Wart A, Chalkley R (2016) FASEB J 30(2): 507-14
    › Primary publication · 26432783 (PubMed) · PMC6188226 (PubMed Central)
  18. A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival. Beckley JR, Chen JS, Yang Y, Peng J, Gould KL (2015) Mol Cell Proteomics 14(12): 3132-41
    › Primary publication · 26412298 (PubMed) · PMC4762628 (PubMed Central)
  19. Regulation of contractile ring formation and septation in Schizosaccharomyces pombe. Willet AH, McDonald NA, Gould KL (2015) Curr Opin Microbiol : 46-52
    › Primary publication · 26340438 (PubMed) · PMC4688203 (PubMed Central)
  20. Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains. Chen JS, Beckley JR, McDonald NA, Ren L, Mangione M, Jang SJ, Elmore ZC, Rachfall N, Feoktistova A, Jones CM, Willet AH, Guillen R, Bitton DA, Bähler J, Jensen MA, Rhind N, Gould KL (2014) G3 (Bethesda) 5(3): 361-70
    › Primary publication · 25552606 (PubMed) · PMC4349090 (PubMed Central)
  21. The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast. Ren L, Willet AH, Roberts-Galbraith RH, McDonald NA, Feoktistova A, Chen JS, Huang H, Guillen R, Boone C, Sidhu SS, Beckley JR, Gould KL (2015) Mol Biol Cell 26(2): 256-69
    › Primary publication · 25428987 (PubMed) · PMC4294673 (PubMed Central)
  22. Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Collier SE, Voehler M, Peng D, Ohi R, Gould KL, Reiter NJ, Ohi MD (2014) Biochemistry 53(41): 6439-51
    › Primary publication · 25263959 (PubMed) · PMC4204884 (PubMed Central)
  23. Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe. Elmore ZC, Beckley JR, Chen JS, Gould KL (2014) G3 (Bethesda) 4(8): 1529-38
    › Primary publication · 24948786 (PubMed) · PMC4132182 (PubMed Central)
  24. An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian cells. Ma H, McLean JR, Gould KL, McCollum D (2014) Methods Mol Biol : 175-91
    › Primary publication · 24943323 (PubMed)
  25. Formin-based control of the actin cytoskeleton during cytokinesis. Bohnert KA, Willet AH, Kovar DR, Gould KL (2013) Biochem Soc Trans 41(6): 1750-4
    › Primary publication · 24256286 (PubMed) · PMC5563852 (PubMed Central)