Free radicals primarily derived from oxygen have been implicated in a wide variety of diseases ranging from atherosclerosis, cancer, neurodegenerative diseases, and even the normal aging process. Free radicals readily attack lipids, proteins, and DNA resulting in mutations and deleterious effects on membrane and protein structure and function.

We discovered a series of novel products of free radical attack on lipids termed isoprostanes. Measurement of isoprostanes has emerged as the most reliable approach to assess free radical reactions in vivo. They also exert potent biological activities, which are currently being characterized. We have also discovered a series of similar compounds (neuroprostanes) formed by free radical attack on a unique lipid in the brain. Their role in neurodegenerative diseases is currently under investigation. Recently, we discovered novel highly reactive molecules that are produced by these pathways. These molecules rapidly adduct to proteins and we are currently exploring the pathobiological consequences of their formation in neurodegenerative and other diseases. More recently, we discovered new products of lipid peroxidation called isofurans whose formation is uniquely regulated by oxygen tension. Measurement of isofurans provides a unique tool to assess the role of oxidative injury in the setting of hyperoxia and in disorders of mitochondrial dysfunction. This provides ample opportunities for scientific investigation in a multidisciplinary area of research that is highly relevant to human disease. .


The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Perioperative intravenous acetaminophen attenuates lipid peroxidation in adults undergoing cardiopulmonary bypass: a randomized clinical trial. Billings FT, Petracek MR, Roberts LJ, Pretorius M (2015) PLoS One 10(2): e0117625
    › Primary publication · 25705899 (PubMed) · PMC4338200 (PubMed Central)
  2. Even free radicals should follow some rules: a guide to free radical research terminology and methodology. Forman HJ, Augusto O, Brigelius-Flohe R, Dennery PA, Kalyanaraman B, Ischiropoulos H, Mann GE, Radi R, Roberts LJ, Vina J, Davies KJ (2015) Free Radic Biol Med : 233-5
    › Primary publication · 25462642 (PubMed)
  3. The isoprostanes--25 years later. Milne GL, Dai Q, Roberts LJ (2015) Biochim Biophys Acta 1851(4): 433-45
    › Primary publication · 25449649 (PubMed) · PMC5404383 (PubMed Central)
  4. Thinking outside the cell: how cell-free hemoglobin can potentiate acute lung injury. Bastarache JA, Roberts LJ, Ware LB (2014) Am J Physiol Lung Cell Mol Physiol 306(3): L231-2
    › Primary publication · 24337924 (PubMed) · PMC3920200 (PubMed Central)
  5. Isoprostane generation and function. Milne GL, Yin H, Hardy KD, Davies SS, Roberts LJ (2011) Chem Rev 111(10): 5973-96
    › Primary publication · 21848345 (PubMed) · PMC3192249 (PubMed Central)
  6. Is there a role for isofurans and neuroprostanes in pre-eclampsia and normal pregnancy? Barden AE, Corcoran TB, Mas E, Durand T, Galano JM, Roberts LJ, Paech M, Muchatuta NA, Phillips M, Mori TA (2012) Antioxid Redox Signal 16(2): 165-9
    › Primary publication · 21827297 (PubMed) · PMC3250920 (PubMed Central)
  7. Hemoglobin attenuates the effects of inspired oxygen on plasma isofurans in humans during upper-limb surgery. Corcoran TB, Barden AE, Mas E, Grape S, Koren V, Phillips M, Roberts LJ, Mori TA (2011) Free Radic Biol Med 51(6): 1235-9
    › Primary publication · 21763419 (PubMed) · PMC3157081 (PubMed Central)
  8. Calciphylaxis in a morbidly obese woman with rheumatoid arthritis presenting with severe weight loss and vitamin D deficiency. Malabu UH, Roberts LJ, Sangla KS (2011) Endocr Pract 17(4): e104-8
    › Primary publication · 21742604 (PubMed)
  9. Treatment with a γ-ketoaldehyde scavenger prevents working memory deficits in hApoE4 mice. Davies SS, Bodine C, Matafonova E, Pantazides BG, Bernoud-Hubac N, Harrison FE, Olson SJ, Montine TJ, Amarnath V, Roberts LJ (2011) J Alzheimers Dis 27(1): 49-59
    › Primary publication · 21709376 (PubMed) · PMC3289064 (PubMed Central)
  10. Are isofurans and neuroprostanes increased after subarachnoid hemorrhage and traumatic brain injury? Corcoran TB, Mas E, Barden AE, Durand T, Galano JM, Roberts LJ, Phillips M, Ho KM, Mori TA (2011) Antioxid Redox Signal 15(10): 2663-7
    › Primary publication · 21702684 (PubMed) · PMC3183650 (PubMed Central)
  11. Oxidative stress measured by urine F2-isoprostane level is associated with prostate cancer. Barocas DA, Motley S, Cookson MS, Chang SS, Penson DF, Dai Q, Milne G, Roberts LJ, Morrow J, Concepcion RS, Smith JA, Fowke JH (2011) J Urol 185(6): 2102-7
    › Primary publication · 21496850 (PubMed) · PMC3093434 (PubMed Central)
  12. Characterization of scavengers of gamma-ketoaldehydes that do not inhibit prostaglandin biosynthesis. Zagol-Ikapitte I, Amarnath V, Bala M, Roberts LJ, Oates JA, Boutaud O (2010) Chem Res Toxicol 23(1): 240-50
    › Primary publication · 20041722 (PubMed) · PMC2831641 (PubMed Central)
  13. Isoketals form cytotoxic phosphatidylethanolamine adducts in cells. Sullivan CB, Matafonova E, Roberts LJ, Amarnath V, Davies SS (2010) J Lipid Res 51(5): 999-1009
    › Primary publication · 19965577 (PubMed) · PMC2853468 (PubMed Central)
  14. Overexpression of Cu/Zn-superoxide dismutase and/or catalase accelerates benzo(a)pyrene detoxification by upregulation of the aryl hydrocarbon receptor in mouse endothelial cells. Wang Z, Yang H, Ramesh A, Roberts LJ, Zhou L, Lin X, Zhao Y, Guo Z (2009) Free Radic Biol Med 47(8): 1221-9
    › Primary publication · 19666105 (PubMed) · PMC2846758 (PubMed Central)