Profile

Cytoskeletal motor proteins generate mechanical forces, which drive numerous cellular processes that are essential for life.  Research in the Tyska laboratory is focused on elucidating the function of the actin cytoskeleton and its associated myosin motor proteins.  The context for our studies is the ‘brush border’: an array of actin-based protrusions known as microvilli, which extend from the surface of polarized epithelial cells.  In the gut, the brush border serves as the sole site of nutrient absorption and a barrier to micro-organisms that reside in the lumenal space.  Using an approach that combines biophysics, biochemistry, and cell biology, we are currently studying mechanisms that control microvillar dynamics, morphology, and function. We have also begun to dissect the mechanism of microvillar assembly, which was jumpstarted by our recent elucidation of the entire brush border proteome.  A broad long-term goal is to develop our understanding of molecules and pathways that may be perturbed in GI diseases characterized by loss of the brush border (e.g. enteropathogenic E. coli infection, celiac disease, and microvillus inclusion disease).

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Shear stress induces noncanonical autophagy in intestinal epithelial monolayers. Kim SW, Ehrman J, Ahn MR, Kondo J, Lopez AAM, Oh YS, Kim XH, Crawley SW, Goldenring JR, Tyska MJ, Rericha EC, Lau KS (2017) Mol Biol Cell 28(22): 3043-3056
    › Primary publication · 28855375 (PubMed) · PMC5662261 (PubMed Central)
  2. Disruption of Rab8a and Rab11a causes formation of basolateral microvilli in neonatal enteropathy. Feng Q, Bonder EM, Engevik AC, Zhang L, Tyska MJ, Goldenring JR, Gao N (2017) J Cell Sci 130(15): 2491-2505
    › Primary publication · 28596241 (PubMed) · PMC5558269 (PubMed Central)
  3. Optimized multiplex immunofluorescence single-cell analysis reveals tuft cell heterogeneity. McKinley ET, Sui Y, Al-Kofahi Y, Millis BA, Tyska MJ, Roland JT, Santamaria-Pang A, Ohland CL, Jobin C, Franklin JL, Lau KS, Gerdes MJ, Coffey RJ (2017) JCI Insight 2(11)
    › Primary publication · 28570279 (PubMed) · PMC5453701 (PubMed Central)
  4. Microtubules regulate brush border formation. Tonucci FM, Ferretti A, Almada E, Cribb P, Vena R, Hidalgo F, Favre C, Tyska MJ, Kaverina I, Larocca MC (2018) J Cell Physiol 233(2): 1468-1480
    › Primary publication · 28548701 (PubMed) · PMC5673559 (PubMed Central)
  5. High-Resolution Image Stitching as a Tool to Assess Tissue-Level Protein Distribution and Localization. Millis BA, Tyska MJ (2017) Methods Mol Biol : 281-296
    › Primary publication · 28502007 (PubMed)
  6. Structure of Myo7b/USH1C complex suggests a general PDZ domain binding mode by MyTH4-FERM myosins. Li J, He Y, Weck ML, Lu Q, Tyska MJ, Zhang M (2017) Proc Natl Acad Sci U S A 114(19): E3776-E3785
    › Primary publication · 28439001 (PubMed) · PMC5441739 (PubMed Central)
  7. Brush Border Destruction by Enterohemorrhagic (EHEC): New Insights From Organoid Culture. Tyska MJ (2016) Cell Mol Gastroenterol Hepatol 2(1): 7-8
    › Primary publication · 28174702 (PubMed) · PMC4980709 (PubMed Central)
  8. MyTH4-FERM myosins in the assembly and maintenance of actin-based protrusions. Weck ML, Grega-Larson NE, Tyska MJ (2017) Curr Opin Cell Biol : 68-78
    › Primary publication · 27836411 (PubMed) · PMC5403704 (PubMed Central)
  9. Myosin-7b Promotes Distal Tip Localization of the Intermicrovillar Adhesion Complex. Weck ML, Crawley SW, Stone CR, Tyska MJ (2016) Curr Biol 26(20): 2717-2728
    › Primary publication · 27666969 (PubMed) · PMC5117816 (PubMed Central)
  10. Impact of the Motor and Tail Domains of Class III Myosins on Regulating the Formation and Elongation of Actin Protrusions. Raval MH, Quintero OA, Weck ML, Unrath WC, Gallagher JW, Cui R, Kachar B, Tyska MJ, Yengo CM (2016) J Biol Chem 291(43): 22781-22792
    › Primary publication · 27582493 (PubMed) · PMC5077211 (PubMed Central)
  11. Patterning protein complexes on DNA nanostructures using a GFP nanobody. Sommese RF, Hariadi RF, Kim K, Liu M, Tyska MJ, Sivaramakrishnan S (2016) Protein Sci 25(11): 2089-2094
    › Primary publication · 27538185 (PubMed) · PMC5079250 (PubMed Central)
  12. Impact of cordon-bleu expression on actin cytoskeleton architecture and dynamics. Grega-Larson NE, Crawley SW, Tyska MJ (2016) Cytoskeleton (Hoboken) 73(11): 670-679
    › Primary publication · 27464680 (PubMed) · PMC5118059 (PubMed Central)
  13. Focal adhesions control cleavage furrow shape and spindle tilt during mitosis. Taneja N, Fenix AM, Rathbun L, Millis BA, Tyska MJ, Hehnly H, Burnette DT (2016) Sci Rep : 29846
    › Primary publication · 27432211 (PubMed) · PMC4949487 (PubMed Central)
  14. Cortactin promotes exosome secretion by controlling branched actin dynamics. Sinha S, Hoshino D, Hong NH, Kirkbride KC, Grega-Larson NE, Seiki M, Tyska MJ, Weaver AM (2016) J Cell Biol 214(2): 197-213
    › Primary publication · 27402952 (PubMed) · PMC4949450 (PubMed Central)
  15. ANKS4B Is Essential for Intermicrovillar Adhesion Complex Formation. Crawley SW, Weck ML, Grega-Larson NE, Shifrin DA, Tyska MJ (2016) Dev Cell 36(2): 190-200
    › Primary publication · 26812018 (PubMed) · PMC4730382 (PubMed Central)
  16. Cordon bleu promotes the assembly of brush border microvilli. Grega-Larson NE, Crawley SW, Erwin AL, Tyska MJ (2015) Mol Biol Cell 26(21): 3803-15
    › Primary publication · 26354418 (PubMed) · PMC4626065 (PubMed Central)
  17. Shaping the intestinal brush border. Crawley SW, Mooseker MS, Tyska MJ (2014) J Cell Biol 207(4): 441-51
    › Primary publication · 25422372 (PubMed) · PMC4242837 (PubMed Central)
  18. Detection of rare antigen-presenting cells through T cell-intrinsic meandering motility, mediated by Myo1g. Gérard A, Patino-Lopez G, Beemiller P, Nambiar R, Ben-Aissa K, Liu Y, Totah FJ, Tyska MJ, Shaw S, Krummel MF (2014) Cell 158(3): 492-505
    › Primary publication · 25083865 (PubMed) · PMC4119593 (PubMed Central)
  19. Dynamics of brush border remodeling induced by enteropathogenic E. coli. Shifrin DA, Crawley SW, Grega-Larson NE, Tyska MJ (2014) Gut Microbes 5(4): 504-16
    › Primary publication · 25076126 (PubMed) · PMC5642117 (PubMed Central)
  20. Apical vesicle trafficking takes center stage in neonatal enteropathies. Knowles BC, Tyska MJ, Goldenring JR (2014) Gastroenterology 147(1): 15-7
    › Primary publication · 24954664 (PubMed)
  21. Myosin Vb uncoupling from RAB8A and RAB11A elicits microvillus inclusion disease. Knowles BC, Roland JT, Krishnan M, Tyska MJ, Lapierre LA, Dickman PS, Goldenring JR, Shub MD (2014) J Clin Invest 124(7): 2947-62
    › Primary publication · 24892806 (PubMed) · PMC4071383 (PubMed Central)
  22. Intestinal brush border assembly driven by protocadherin-based intermicrovillar adhesion. Crawley SW, Shifrin DA, Grega-Larson NE, McConnell RE, Benesh AE, Mao S, Zheng Y, Zheng QY, Nam KT, Millis BA, Kachar B, Tyska MJ (2014) Cell 157(2): 433-446
    › Primary publication · 24725409 (PubMed) · PMC3992856 (PubMed Central)
  23. Motor and tail homology 1 (Th1) domains antagonistically control myosin-1 dynamics. Mazerik JN, Kraft LJ, Kenworthy AK, Tyska MJ (2014) Biophys J 106(3): 649-58
    › Primary publication · 24507605 (PubMed) · PMC3944834 (PubMed Central)
  24. Exosome secretion is enhanced by invadopodia and drives invasive behavior. Hoshino D, Kirkbride KC, Costello K, Clark ES, Sinha S, Grega-Larson N, Tyska MJ, Weaver AM (2013) Cell Rep 5(5): 1159-68
    › Primary publication · 24290760 (PubMed) · PMC3873329 (PubMed Central)
  25. Extracellular vesicles: communication, coercion, and conditioning. Shifrin DA, Demory Beckler M, Coffey RJ, Tyska MJ (2013) Mol Biol Cell 24(9): 1253-9
    › Primary publication · 23630232 (PubMed) · PMC3639038 (PubMed Central)
  26. Membrane-bound myo1c powers asymmetric motility of actin filaments. Pyrpassopoulos S, Feeser EA, Mazerik JN, Tyska MJ, Ostap EM (2012) Curr Biol 22(18): 1688-92
    › Primary publication · 22863317 (PubMed) · PMC3461085 (PubMed Central)
  27. Ready…aim…fire into the lumen: a new role for enterocyte microvilli in gut host defense. Shifrin DA, Tyska MJ (2012) Gut Microbes 3(5): 460-2
    › Primary publication · 22825496 (PubMed) · PMC3466500 (PubMed Central)
  28. Enterocyte microvillus-derived vesicles detoxify bacterial products and regulate epithelial-microbial interactions. Shifrin DA, McConnell RE, Nambiar R, Higginbotham JN, Coffey RJ, Tyska MJ (2012) Curr Biol 22(7): 627-31
    › Primary publication · 22386311 (PubMed) · PMC3326206 (PubMed Central)
  29. Myosin-1A targets to microvilli using multiple membrane binding motifs in the tail homology 1 (TH1) domain. Mazerik JN, Tyska MJ (2012) J Biol Chem 287(16): 13104-15
    › Primary publication · 22367206 (PubMed) · PMC3339983 (PubMed Central)
  30. Expression and localization of myosin-1d in the developing nervous system. Benesh AE, Fleming JT, Chiang C, Carter BD, Tyska MJ (2012) Brain Res : 9-22
    › Primary publication · 22284616 (PubMed) · PMC3278530 (PubMed Central)
  31. Constitutively active ezrin increases membrane tension, slows migration, and impedes endothelial transmigration of lymphocytes in vivo in mice. Liu Y, Belkina NV, Park C, Nambiar R, Loughhead SM, Patino-Lopez G, Ben-Aissa K, Hao JJ, Kruhlak MJ, Qi H, von Andrian UH, Kehrl JH, Tyska MJ, Shaw S (2012) Blood 119(2): 445-53
    › Primary publication · 22106344 (PubMed) · PMC3257010 (PubMed Central)
  32. A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors. Pyburn TM, Bensing BA, Xiong YQ, Melancon BJ, Tomasiak TM, Ward NJ, Yankovskaya V, Oliver KM, Cecchini G, Sulikowski GA, Tyska MJ, Sullam PM, Iverson TM (2011) PLoS Pathog 7(7): e1002112
    › Primary publication · 21765814 (PubMed) · PMC3131266 (PubMed Central)
  33. Amphiregulin exosomes increase cancer cell invasion. Higginbotham JN, Demory Beckler M, Gephart JD, Franklin JL, Bogatcheva G, Kremers GJ, Piston DW, Ayers GD, McConnell RE, Tyska MJ, Coffey RJ (2011) Curr Biol 21(9): 779-86
    › Primary publication · 21514161 (PubMed) · PMC3417320 (PubMed Central)
  34. Proteomic analysis of the enterocyte brush border. McConnell RE, Benesh AE, Mao S, Tabb DL, Tyska MJ (2011) Am J Physiol Gastrointest Liver Physiol 300(5): G914-26
    › Primary publication · 21330445 (PubMed) · PMC3094140 (PubMed Central)
  35. Myosin-1a: A motor for microvillar membrane movement and mechanics. Tyska MJ, Nambiar R (2010) Commun Integr Biol 3(1): 64-6
    › Primary publication · 20539787 (PubMed) · PMC2881245 (PubMed Central)
  36. Leveraging the membrane - cytoskeleton interface with myosin-1. McConnell RE, Tyska MJ (2010) Trends Cell Biol 20(7): 418-26
    › Primary publication · 20471271 (PubMed) · PMC2897960 (PubMed Central)
  37. Myosin motor function: the ins and outs of actin-based membrane protrusions. Nambiar R, McConnell RE, Tyska MJ (2010) Cell Mol Life Sci 67(8): 1239-54
    › Primary publication · 20107861 (PubMed) · PMC3095969 (PubMed Central)
  38. Differential localization and dynamics of class I myosins in the enterocyte microvillus. Benesh AE, Nambiar R, McConnell RE, Mao S, Tabb DL, Tyska MJ (2010) Mol Biol Cell 21(6): 970-8
    › Primary publication · 20089841 (PubMed) · PMC2836977 (PubMed Central)
  39. Control of cell membrane tension by myosin-I. Nambiar R, McConnell RE, Tyska MJ (2009) Proc Natl Acad Sci U S A 106(29): 11972-7
    › Primary publication · 19574460 (PubMed) · PMC2715533 (PubMed Central)
  40. The enterocyte microvillus is a vesicle-generating organelle. McConnell RE, Higginbotham JN, Shifrin DA, Tabb DL, Coffey RJ, Tyska MJ (2009) J Cell Biol 185(7): 1285-98
    › Primary publication · 19564407 (PubMed) · PMC2712962 (PubMed Central)
  41. Differential gene expression in normal esophagus and Barrett's esophagus. Wang J, Qin R, Ma Y, Wu H, Peters H, Tyska M, Shaheen NJ, Chen X (2009) J Gastroenterol 44(9): 897-911
    › Primary publication · 19468668 (PubMed) · PMC3811010 (PubMed Central)
  42. Human deafness mutation E385D disrupts the mechanochemical coupling and subcellular targeting of myosin-1a. Yengo CM, Ananthanarayanan SK, Brosey CA, Mao S, Tyska MJ (2008) Biophys J 94(2): L5-7
    › Primary publication · 17981900 (PubMed) · PMC2157235 (PubMed Central)
  43. Myosin-1a powers the sliding of apical membrane along microvillar actin bundles. McConnell RE, Tyska MJ (2007) J Cell Biol 177(4): 671-81
    › Primary publication · 17502425 (PubMed) · PMC2064212 (PubMed Central)
  44. Myosin at work: motor adaptations for a variety of cellular functions. O'Connell CB, Tyska MJ, Mooseker MS (2007) Biochim Biophys Acta 1773(5): 615-30
    › Primary publication · 16904206 (PubMed)
  45. Myosin-1a is critical for normal brush border structure and composition. Tyska MJ, Mackey AT, Huang JD, Copeland NG, Jenkins NA, Mooseker MS (2005) Mol Biol Cell 16(5): 2443-57
    › Primary publication · 15758024 (PubMed) · PMC1087248 (PubMed Central)
  46. A role for myosin-1A in the localization of a brush border disaccharidase. Tyska MJ, Mooseker MS (2004) J Cell Biol 165(3): 395-405
    › Primary publication · 15138292 (PubMed) · PMC2172191 (PubMed Central)
  47. Myosin-V motility: these levers were made for walking. Tyska MJ, Mooseker MS (2003) Trends Cell Biol 13(9): 447-51
    › Primary publication · 12946621 (PubMed)
  48. MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells. Tyska MJ, Mooseker MS (2002) Biophys J 82(4): 1869-83
    › Primary publication · 11916846 (PubMed) · PMC1301984 (PubMed Central)
  49. The myosin power stroke. Tyska MJ, Warshaw DM (2002) Cell Motil Cytoskeleton 51(1): 1-15
    › Primary publication · 11810692 (PubMed)
  50. Myosin-IXb is a single-headed and processive motor. Post PL, Tyska MJ, O'Connell CB, Johung K, Hayward A, Mooseker MS (2002) J Biol Chem 277(14): 11679-83
    › Primary publication · 11801597 (PubMed)
  51. The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors. Reck-Peterson SL, Tyska MJ, Novick PJ, Mooseker MS (2001) J Cell Biol 153(5): 1121-6
    › Primary publication · 11381095 (PubMed) · PMC2174330 (PubMed Central)
  52. R403Q and L908V mutant beta-cardiac myosin from patients with familial hypertrophic cardiomyopathy exhibit enhanced mechanical performance at the single molecule level. Palmiter KA, Tyska MJ, Haeberle JR, Alpert NR, Fananapazir L, Warshaw DM (2000) J Muscle Res Cell Motil 21(7): 609-20
    › Primary publication · 11227787 (PubMed)
  53. The light chain binding domain of expressed smooth muscle heavy meromyosin acts as a mechanical lever. Warshaw DM, Guilford WH, Freyzon Y, Krementsova E, Palmiter KA, Tyska MJ, Baker JE, Trybus KM (2000) J Biol Chem 275(47): 37167-72
    › Primary publication · 10945998 (PubMed)
  54. Functional consequences of mutations in the smooth muscle myosin heavy chain at sites implicated in familial hypertrophic cardiomyopathy. Yamashita H, Tyska MJ, Warshaw DM, Lowey S, Trybus KM (2000) J Biol Chem 275(36): 28045-52
    › Primary publication · 10882745 (PubMed)
  55. Single-molecule mechanics of R403Q cardiac myosin isolated from the mouse model of familial hypertrophic cardiomyopathy. Tyska MJ, Hayes E, Giewat M, Seidman CE, Seidman JG, Warshaw DM (2000) Circ Res 86(7): 737-44
    › Primary publication · 10764406 (PubMed)
  56. Kinetic differences at the single molecule level account for the functional diversity of rabbit cardiac myosin isoforms. Palmiter KA, Tyska MJ, Dupuis DE, Alpert NR, Warshaw DM (1999) J Physiol : 669-78
    › Primary publication · 10457082 (PubMed) · PMC2269540 (PubMed Central)
  57. Two heads of myosin are better than one for generating force and motion. Tyska MJ, Dupuis DE, Guilford WH, Patlak JB, Waller GS, Trybus KM, Warshaw DM, Lowey S (1999) Proc Natl Acad Sci U S A 96(8): 4402-7
    › Primary publication · 10200274 (PubMed) · PMC16344 (PubMed Central)
  58. A 7-amino-acid insert in the heavy chain nucleotide binding loop alters the kinetics of smooth muscle myosin in the laser trap. Lauzon AM, Tyska MJ, Rovner AS, Freyzon Y, Warshaw DM, Trybus KM (1998) J Muscle Res Cell Motil 19(8): 825-37
    › Primary publication · 10047983 (PubMed)