The work in our laboratory has focused on the commitment, differentiation and function of cardiac and vascular cells in the embryo. The early heart development and vasculogenesis provide excellent systems to examine fundamental issues in developmental biology. Heart development involves the commitment of embryogenic cells to the cardiogenic mesodermal lineage, coordinated activation of cell-specific genes in committed cells, and the diversification of progenitors in a highly patterned manner leading to the generation of distinct myogenic phenotypes. Our current work is focused on the function of two genes that were discovered in our laboratory and analysis of the conserved system whereby coelomates generate vessels to organs during development. LEK1 is a large and complex protein that regulates proliferation and differentiation of cardiac myocytes. Through a series of biochemical, morphological, genetic and cell biological experiments, we have determined that LEK1 proteins function in cell movement, trafficking and division through its interaction with Rb proteins, the cytoskeleton and the SNARE complex. Our ongoing work is focused on conditionally inhibiting the function of this gene in developing mice and continuing our studies of LEK1 interaction with subcellular domains. Bves is an integral membrane protein discovered by former members of the lab. This protein is essential for proper cell/cell interaction during coronary vessel development. Our work has shown that Bves is one of the first proteins trafficked to points of cell/cell contact and that it associates with cytoplasmic proteins that regulate cell/cell adhesion, process formation and movement. Our goal is to determine how disruption of Bves function impacts embryonic development in vertebrate and invertebrate development. Lastly, we have determined that blood vessel formation to internal organs has a conserved program that is been overlooked heretofore. We have discovered that vasculogenesis to the heart and gut is linked to the formation of the embryonic coelom and that progenitors of these blood vessels arise via a mechanism unlike that of the body wall and limbs. We are now disrupting this developmental system in an effort to understand the cellular and molecular mechanisms driving this process.


The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities. Pfaltzgraff ER, Roth GM, Miller PM, Gintzig AG, Ohi R, Bader DM (2016) Mol Biol Cell 27(13): 1990-9
    › Primary publication · 27146114 (PubMed) · PMC4927273 (PubMed Central)
  2. BVES Regulates Intestinal Stem Cell Programs and Intestinal Crypt Viability after Radiation. Reddy VK, Short SP, Barrett CW, Mittal MK, Keating CE, Thompson JJ, Harris EI, Revetta F, Bader DM, Brand T, Washington MK, Williams CS (2016) Stem Cells 34(6): 1626-36
    › Primary publication · 26891025 (PubMed) · PMC4893006 (PubMed Central)
  3. Interprofessional projects promote and strengthen interdisciplinary collaboration. Pfaltzgraff ER, Samade R, Adams R, Levic DS, Bader DM, Fleming AE (2015) Med Educ 49(11): 1156-7
    › Primary publication · 26494089 (PubMed) · PMC4680992 (PubMed Central)
  4. The kinetochore protein, CENPF, is mutated in human ciliopathy and microcephaly phenotypes. Waters AM, Asfahani R, Carroll P, Bicknell L, Lescai F, Bright A, Chanudet E, Brooks A, Christou-Savina S, Osman G, Walsh P, Bacchelli C, Chapgier A, Vernay B, Bader DM, Deshpande C, O' Sullivan M, Ocaka L, Stanescu H, Stewart HS, Hildebrandt F, Otto E, Johnson CA, Szymanska K, Katsanis N, Davis E, Kleta R, Hubank M, Doxsey S, Jackson A, Stupka E, Winey M, Beales PL (2015) J Med Genet 52(3): 147-56
    › Primary publication · 25564561 (PubMed) · PMC4345935 (PubMed Central)
  5. Heterogeneity in vascular smooth muscle cell embryonic origin in relation to adult structure, physiology, and disease. Pfaltzgraff ER, Bader DM (2015) Dev Dyn 244(3): 410-6
    › Primary publication · 25546231 (PubMed) · PMC4344868 (PubMed Central)
  6. Isolation and physiological analysis of mouse cardiomyocytes. Roth GM, Bader DM, Pfaltzgraff ER (2014) J Vis Exp (91): e51109
    › Primary publication · 25225886 (PubMed) · PMC4828048 (PubMed Central)
  7. Resident progenitors, not exogenous migratory cells, generate the majority of visceral mesothelium in organogenesis. Winters NI, Williams AM, Bader DM (2014) Dev Biol 391(2): 125-32
    › Primary publication · 24746591 (PubMed) · PMC4037704 (PubMed Central)
  8. Embryonic domains of the aorta derived from diverse origins exhibit distinct properties that converge into a common phenotype in the adult. Pfaltzgraff ER, Shelton EL, Galindo CL, Nelms BL, Hooper CW, Poole SD, Labosky PA, Bader DM, Reese J (2014) J Mol Cell Cardiol : 88-96
    › Primary publication · 24508561 (PubMed) · PMC4034360 (PubMed Central)
  9. Bves and NDRG4 regulate directional epicardial cell migration through autocrine extracellular matrix deposition. Benesh EC, Miller PM, Pfaltzgraff ER, Grega-Larson NE, Hager HA, Sung BH, Qu X, Baldwin HS, Weaver AM, Bader DM (2013) Mol Biol Cell 24(22): 3496-510
    › Primary publication · 24048452 (PubMed) · PMC3826988 (PubMed Central)
  10. Autotaxin signaling governs phenotypic heterogeneity in visceral and parietal mesothelia. Shelton EL, Galindo CL, Williams CH, Pfaltzgraff E, Hong CC, Bader DM (2013) PLoS One 8(7): e69712
    › Primary publication · 23936085 (PubMed) · PMC3723636 (PubMed Central)
  11. Thymosin β4 mobilizes mesothelial cells for blood vessel repair. Shelton EL, Bader DM (2012) Ann N Y Acad Sci : 125-30
    › Primary publication · 23045980 (PubMed) · PMC3693742 (PubMed Central)
  12. Comprehensive timeline of mesodermal development in the quail small intestine. Thomason RT, Bader DM, Winters NI (2012) Dev Dyn 241(11): 1678-94
    › Primary publication · 22930586 (PubMed) · PMC3475750 (PubMed Central)
  13. Identification of a novel developmental mechanism in the generation of mesothelia. Winters NI, Thomason RT, Bader DM (2012) Development 139(16): 2926-34
    › Primary publication · 22764055 (PubMed) · PMC3403102 (PubMed Central)
  14. Cardiac-specific deletion of the microtubule-binding protein CENP-F causes dilated cardiomyopathy. Dees E, Miller PM, Moynihan KL, Pooley RD, Hunt RP, Galindo CL, Rottman JN, Bader DM (2012) Dis Model Mech 5(4): 468-80
    › Primary publication · 22563055 (PubMed) · PMC3380710 (PubMed Central)
  15. Omental grafting: a cell-based therapy for blood vessel repair. Shelton EL, Poole SD, Reese J, Bader DM (2013) J Tissue Eng Regen Med 7(6): 421-33
    › Primary publication · 22318999 (PubMed) · PMC3672266 (PubMed Central)
  16. Application of small organic molecules reveals cooperative TGFβ and BMP regulation of mesothelial cell behaviors. Cross EE, Thomason RT, Martinez M, Hopkins CR, Hong CC, Bader DM (2011) ACS Chem Biol 6(9): 952-61
    › Primary publication · 21740033 (PubMed) · PMC3177035 (PubMed Central)
  17. Bves modulates tight junction associated signaling. Russ PK, Pino CJ, Williams CS, Bader DM, Haselton FR, Chang MS (2011) PLoS One 6(1): e14563
    › Primary publication · 21283798 (PubMed) · PMC3024319 (PubMed Central)
  18. Commitment and differentiation of cardiac myocytes. Litvin J, Montgomery M, Gonzalez-Sanchez A, Bisaha JG, Bader D (1992) Trends Cardiovasc Med 2(1): 27-32
    › Primary publication · 21239285 (PubMed)
  19. Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome. Moynihan KL, Pooley R, Miller PM, Kaverina I, Bader DM (2009) Mol Biol Cell 20(22): 4790-803
    › Primary publication · 19793914 (PubMed) · PMC2777108 (PubMed Central)
  20. Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development. Que J, Wilm B, Hasegawa H, Wang F, Bader D, Hogan BL (2008) Proc Natl Acad Sci U S A 105(43): 16626-30
    › Primary publication · 18922767 (PubMed) · PMC2567908 (PubMed Central)
  21. Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport. Pooley RD, Moynihan KL, Soukoulis V, Reddy S, Francis R, Lo C, Ma LJ, Bader DM (2008) J Cell Sci 121(Pt 20): 3413-21
    › Primary publication · 18827011 (PubMed) · PMC2849733 (PubMed Central)
  22. Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity. Smith TK, Hager HA, Francis R, Kilkenny DM, Lo CW, Bader DM (2008) Proc Natl Acad Sci U S A 105(24): 8298-303
    › Primary publication · 18541910 (PubMed) · PMC2423412 (PubMed Central)
  23. Signals from both sides: Control of cardiac development by the endocardium and epicardium. Smith TK, Bader DM (2007) Semin Cell Dev Biol 18(1): 84-9
    › Primary publication · 17267246 (PubMed) · PMC2849752 (PubMed Central)
  24. Specific deletion of CMF1 nuclear localization domain causes incomplete cell cycle withdrawal and impaired differentiation in avian skeletal myoblasts. Dees E, Robertson JB, Zhu T, Bader D (2006) Exp Cell Res 312(16): 3000-14
    › Primary publication · 16904105 (PubMed)
  25. CytLEK1 is a regulator of plasma membrane recycling through its interaction with SNAP-25. Pooley RD, Reddy S, Soukoulis V, Roland JT, Goldenring JR, Bader DM (2006) Mol Biol Cell 17(7): 3176-86
    › Primary publication · 16672379 (PubMed) · PMC1483049 (PubMed Central)
  26. Characterization of Bves expression during mouse development using newly generated immunoreagents. Smith TK, Bader DM (2006) Dev Dyn 235(6): 1701-8
    › Primary publication · 16538658 (PubMed) · PMC4678624 (PubMed Central)
  27. Bves, a member of the Popeye domain-containing gene family. Osler ME, Smith TK, Bader DM (2006) Dev Dyn 235(3): 586-93
    › Primary publication · 16444674 (PubMed) · PMC2849751 (PubMed Central)
  28. Xbves is a regulator of epithelial movement during early Xenopus laevis development. Ripley AN, Osler ME, Wright CV, Bader D (2006) Proc Natl Acad Sci U S A 103(3): 614-9
    › Primary publication · 16407138 (PubMed) · PMC1334639 (PubMed Central)
  29. The serosal mesothelium is a major source of smooth muscle cells of the gut vasculature. Wilm B, Ipenberg A, Hastie ND, Burch JB, Bader DM (2005) Development 132(23): 5317-28
    › Primary publication · 16284122 (PubMed)
  30. Bves modulates epithelial integrity through an interaction at the tight junction. Osler ME, Chang MS, Bader DM (2005) J Cell Sci 118(Pt 20): 4667-78
    › Primary publication · 16188940 (PubMed)
  31. LEK1 protein expression in normal and dysregulated cardiomyocyte mitosis. Dees E, Robertson JB, Ashe M, Pabón-Peña LM, Bader D, Goodwin RL (2005) Anat Rec A Discov Mol Cell Evol Biol 286(1): 823-32
    › Primary publication · 16047383 (PubMed)
  32. Cytoplasmic LEK1 is a regulator of microtubule function through its interaction with the LIS1 pathway. Soukoulis V, Reddy S, Pooley RD, Feng Y, Walsh CA, Bader DM (2005) Proc Natl Acad Sci U S A 102(24): 8549-54
    › Primary publication · 15939891 (PubMed) · PMC1150833 (PubMed Central)
  33. Bves is expressed in the epithelial components of the retina, lens, and cornea. Ripley AN, Chang MS, Bader DM (2004) Invest Ophthalmol Vis Sci 45(8): 2475-83
    › Primary publication · 15277466 (PubMed)
  34. Bves expression during avian embryogenesis. Osler ME, Bader DM (2004) Dev Dyn 229(3): 658-67
    › Primary publication · 14991721 (PubMed)
  35. LEK1 is a potential inhibitor of pocket protein-mediated cellular processes. Ashe M, Pabon-Peña L, Dees E, Price KL, Bader D (2004) J Biol Chem 279(1): 664-76
    › Primary publication · 14555653 (PubMed)
  36. Coronary vessel development: a unique form of vasculogenesis. Wada AM, Willet SG, Bader D (2003) Arterioscler Thromb Vasc Biol 23(12): 2138-45
    › Primary publication · 14525796 (PubMed)
  37. Membrane topology of Bves/Pop1A, a cell adhesion molecule that displays dynamic changes in cellular distribution during development. Knight RF, Bader DM, Backstrom JR (2003) J Biol Chem 278(35): 32872-9
    › Primary publication · 12815060 (PubMed)
  38. Epicardial/Mesothelial cell line retains vasculogenic potential of embryonic epicardium. Wada AM, Smith TK, Osler ME, Reese DE, Bader DM (2003) Circ Res 92(5): 525-31
    › Primary publication · 12600887 (PubMed)
  39. Development of the coronary vessel system. Reese DE, Mikawa T, Bader DM (2002) Circ Res 91(9): 761-8
    › Primary publication · 12411389 (PubMed)
  40. Hole is a novel gene product expressed in the developing heart and brain. Nesset AL, Bader DM (2002) Mech Dev 117(1-2): 347-50
    › Primary publication · 12204283 (PubMed)
  41. Bves: prototype of a new class of cell adhesion molecules expressed during coronary artery development. Wada AM, Reese DE, Bader DM (2001) Development 128(11): 2085-93
    › Primary publication · 11493530 (PubMed)
  42. Characterization of CMF1 in avian skeletal muscle. Dees E, Pabón-Peña LM, Goodwin RL, Bader D (2000) Dev Dyn 219(2): 169-81
    › Primary publication · 11002337 (PubMed)
  43. Analysis of CMF1 reveals a bone morphogenetic protein-independent component of the cardiomyogenic pathway. Pabón-Peña LM, Goodwin RL, Cise LJ, Bader D (2000) J Biol Chem 275(28): 21453-9
    › Primary publication · 10747923 (PubMed)
  44. Identification and genomic cloning of CMHC1. A unique myosin heavy chain expressed exclusively in the developing chicken heart. Croissant JD, Carpenter S, Bader D (2000) J Biol Chem 275(3): 1944-51
    › Primary publication · 10636896 (PubMed)
  45. Cloning and expression of hbves, a novel and highly conserved mRNA expressed in the developing and adult heart and skeletal muscle in the human. Reese DE, Bader DM (1999) Mamm Genome 10(9): 913-5
    › Primary publication · 10441744 (PubMed)
  46. The cloning and analysis of LEK1 identifies variations in the LEK/centromere protein F/mitosin gene family. Goodwin RL, Pabón-Peña LM, Foster GC, Bader D (1999) J Biol Chem 274(26): 18597-604
    › Primary publication · 10373470 (PubMed)
  47. bves: A novel gene expressed during coronary blood vessel development. Reese DE, Zavaljevski M, Streiff NL, Bader D (1999) Dev Biol 209(1): 159-71
    › Primary publication · 10208750 (PubMed)
  48. Avian cardiac progenitors: methods for isolation, culture, and analysis of differentiation. Gannon M, Bader D (1997) Methods Cell Biol : 117-32
    › Primary publication · 9379947 (PubMed)
  49. Molecular cloning and expression of two novel avian cytochrome P450 1A enzymes induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Gilday D, Gannon M, Yutzey K, Bader D, Rifkind AB (1996) J Biol Chem 271(51): 33054-9
    › Primary publication · 8955152 (PubMed)
  50. Identification of a novel cardiac-specific transcript critical for cardiac myocyte differentiation. Wei Y, Bader D, Litvin J (1996) Development 122(9): 2779-89
    › Primary publication · 8787752 (PubMed)
  51. Identification of DNA-binding protein(s) in the developing heart. Litvin J, Montgomery MO, Goldhamer DJ, Emerson CP, Bader DM (1993) Dev Biol 156(2): 409-17
    › Primary publication · 8462740 (PubMed)
  52. Staging of commitment and differentiation of avian cardiac myocytes. Montgomery MO, Litvin J, Gonzalez-Sanchez A, Bader D (1994) Dev Biol 164(1): 63-71
    › Primary publication · 8026637 (PubMed)
  53. QCE-6: a clonal cell line with cardiac myogenic and endothelial cell potentials. Eisenberg CA, Bader D (1995) Dev Biol 167(2): 469-81
    › Primary publication · 7875372 (PubMed)
  54. Commitment, differentiation, and diversification of avian cardiac progenitor cells. Melnik N, Yutzey KE, Gannon M, Bader D (1995) Ann N Y Acad Sci : 1-8
    › Primary publication · 7755245 (PubMed)
  55. Initiation of cardiac differentiation occurs in the absence of anterior endoderm. Gannon M, Bader D (1995) Development 121(8): 2439-50
    › Primary publication · 7671808 (PubMed)
  56. Diversification of cardiomyogenic cell lineages in vitro. Yutzey K, Gannon M, Bader D (1995) Dev Biol 170(2): 531-41
    › Primary publication · 7649381 (PubMed)
  57. Diversification of cardiomyogenic cell lineages during early heart development. Yutzey KE, Bader D (1995) Circ Res 77(2): 216-9
    › Primary publication · 7614708 (PubMed)
  58. Expression of the atrial-specific myosin heavy chain AMHC1 and the establishment of anteroposterior polarity in the developing chicken heart. Yutzey KE, Rhee JT, Bader D (1994) Development 120(4): 871-83
    › Primary publication · 7600964 (PubMed)
  59. Reinnervation of motor endplate-containing and motor endplate-less muscle grafts. Bader D (1980) Dev Biol 77(2): 315-27
    › Primary publication · 7399124 (PubMed)
  60. Density and distribution of alpha-bungarotoxin-binding sites in postsynaptic structures of regenerated rat skeletal muscle. Bader D (1981) J Cell Biol 88(2): 338-45
    › Primary publication · 7204497 (PubMed) · PMC2111735 (PubMed Central)
  61. Restoration of full mass in nerve-intact muscle grafts after delayed reinnervation. Carlson BM, Foster AH, Bader DM, Hník P, Vejsada R (1983) Experientia 39(2): 171-2
    › Primary publication · 6832293 (PubMed)
  62. Immunochemical analysis of myosin heavy chains in the developing chicken heart. González-Sánchez A, Bader D (1984) Dev Biol 103(1): 151-8
    › Primary publication · 6370758 (PubMed)
  63. Comparison between grafts with intact nerves and standard free grafts of the rat extensor digitorum longus muscle. Carlson BM, Hník P, Tucek S, Vejsada R, Bader DM, Faulkner JA (1981) Physiol Bohemoslov 30(6): 505-14
    › Primary publication · 6275429 (PubMed)
  64. Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro. Bader D, Masaki T, Fischman DA (1982) J Cell Biol 95(3): 763-70
    › Primary publication · 6185504 (PubMed) · PMC2112936 (PubMed Central)
  65. Immunochemical analysis of C-protein isoform transitions during the development of chicken skeletal muscle. Obinata T, Reinach FC, Bader DM, Masaki T, Kitani S, Fischman DA (1984) Dev Biol 101(1): 116-24
    › Primary publication · 6141116 (PubMed)
  66. Characterization of a myosin heavy chain in the conductive system of the adult and developing chicken heart. González-Sánchez A, Bader D (1985) J Cell Biol 100(1): 270-5
    › Primary publication · 3880754 (PubMed) · PMC2113473 (PubMed Central)
  67. Detection of a ventricular-specific myosin heavy chain in adult and developing chicken heart. Zhang Y, Shafiq SA, Bader D (1986) J Cell Biol 102(4): 1480-4
    › Primary publication · 3514633 (PubMed) · PMC2114157 (PubMed Central)
  68. Regulating expression of protein isoforms. Overview. Fischman DA, Bader D, Obinata T (1985) Adv Exp Med Biol : 203-14
    › Primary publication · 2860776 (PubMed)
  69. Molecular cloning and expression of chicken cardiac troponin C. Toyota N, Shimada Y, Bader D (1989) Circ Res 65(5): 1241-6
    › Primary publication · 2805242 (PubMed)
  70. Myosin heavy chain expression in embryonic cardiac cell cultures. Zadeh BJ, González-Sánchez A, Fischman DA, Bader DM (1986) Dev Biol 115(1): 204-14
    › Primary publication · 2422070 (PubMed)
  71. Identification and characterization of a ventricular-specific avian myosin heavy chain, VMHC1: expression in differentiating cardiac and skeletal muscle. Bisaha JG, Bader D (1991) Dev Biol 148(1): 355-64
    › Primary publication · 1936571 (PubMed)
  72. Structure and developmental expression of troponin I isoforms. cDNA clone analysis of avian cardiac troponin I mRNA. Hastings KE, Koppe RI, Marmor E, Bader D, Shimada Y, Toyota N (1991) J Biol Chem 266(29): 19659-65
    › Primary publication · 1918073 (PubMed)
  73. Expression of sarcomeric myosin in the presumptive myocardium of chicken embryos occurs within six hours of myocyte commitment. Han Y, Dennis JE, Cohen-Gould L, Bader DM, Fischman DA (1992) Dev Dyn 193(3): 257-65
    › Primary publication · 1600244 (PubMed)
  74. Myoblast therapy. Epstein HF, Fischman DA, Bader D, Changeux JP, Buckhold K, Ordahl CP, Hoffman E, Kedes LH, Konieczny S, Leinwand LA (1992) Science 257(5071): 738
    › Primary publication · 1496388 (PubMed)
  75. Repair and reorganization of minced cardiac muscle in the adult newt (Notophthalmus viridescens). Bader D, Oberpriller JO (1978) J Morphol 155(3): 349-57
    › Primary publication · 633377 (PubMed)
  76. Autoradiographic and electron microscopic studies of minced cardiac muscle regeneration in the adult newt, notophthalmus viridescens. Bader D, Oberpriller J (1979) J Exp Zool 208(2): 177-93
    › Primary publication · 469482 (PubMed)