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The mission of the Vanderbilt Transgenic Mouse/Embryonic Stem Cell Shared Resource (TMESCSR) is to provide services that facilitate the generation, storage and regeneration of genetically altered mice. We provide a wide array of services that are essential for the derivation, maintenance and long-term storage of genetically altered mouse models.  

The following procedures are provided on a fee-for-service basis:

  • Gene targeting in mouse ES cells
  • Pronuclear DNA microinjections
  • Recombinase Mediated Cassette Exchange
  • Embryo and sperm cryopreservation
  • In vitro fertilization and rederivation
  • BAC recombineering
  • Southern Blot Hybridization

The TMESCSR is staffed by experienced stem cell scientists and highly competent staff, and our track record in performing these services is extensive.  In addition, we are also happy to work with you to develop and/or implement new methods and techniques.  

  • Location: 9410 MRB-IV
  • Phone: 615-936-3454  


CRISPR is a new system that efficiently allows targeted mutagenesis and genome editing in mouse embryos.  We’ve created CRISPR/Cas9 guidelines and updated our services to integrate this technology into our suite of tools.    

CRISPR/Cas9 Guidelines for TMESCSR users

Results example. See data from a recent CRISPR mutagenesis experiment:

CRISPR/Cas9 Services page + data example


Pronuclear DNA microinjection - Watch video

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