The mission of the Vanderbilt Transgenic Mouse/Embryonic Stem Cell Shared Resource (TMESCSR) is to provide services that facilitate the generation, storage and regeneration of genetically altered mice. We provide a wide array of services that are essential for the derivation, maintenance and long-term storage of genetically altered mouse models.  

The following procedures are provided on a fee-for-service basis:

  • Gene targeting in mouse ES cells
  • Pronuclear DNA microinjections
  • Recombinase Mediated Cassette Exchange
  • Embryo and sperm cryopreservation
  • In vitro fertilization and rederivation
  • BAC recombineering
  • Southern Blot Hybridization

The TMESCSR is staffed by experienced stem cell scientists and highly competent staff, and our track record in performing these services is extensive.  In addition, we are also happy to work with you to develop and/or implement new methods and techniques.  

  • Location: 9410 MRB-IV
  • Phone: 615-936-3454  

NEW!  CRISPR/CAS9 SERVICES AND GUIDELINES 

CRISPR is a new system that efficiently allows targeted mutagenesis and genome editing in mouse embryos.  We’ve created CRISPR/Cas9 guidelines and updated our services to integrate this technology into our suite of tools.    

CRISPR/Cas9 Guidelines for TMESCSR users

Results example. See data from a recent CRISPR mutagenesis experiment:

CRISPR/Cas9 Services page + data example

Publications/Citations

Featured publications

  1. Targeted colonic claudin-2 expression renders resistance to epithelial injury, induces immune suppression, and protects from colitis. Ahmad R, Chaturvedi R, Olivares-Villagómez D, Habib T, Asim M, Shivesh P, Polk DB, Wilson KT, Washington MK, Van Kaer L, Dhawan P, Singh AB (2014) Mucosal Immunol
    › Citation · 24670427 (PubMed)
  2. Type 2 Diabetes and Congenital Hyperinsulinism Cause DNA Double-Strand Breaks and p53 Activity in β Cells. Tornovsky-Babeay S, Dadon D, Ziv O, Tzipilevich E, Kadosh T, Schyr-Ben Haroush R, Hija A, Stolovich-Rain M, Furth-Lavi J, Granot Z, Porat S, Philipson LH, Herold KC, Bhatti TR, Stanley C, Ashcroft FM, In't Veld P, Saada A, Magnuson MA, Glaser B, Dor Y (2014) Cell Metab 19(1): 109-21
    › Citation · 24332968 (PubMed)
  3. Modulation of behavioral networks by selective interneuronal inactivation. Schmidt MJ, Horvath S, Ebert P, Norris JL, Seeley EH, Brown J, Gellert L, Everheart M, Garbett KA, Grice TW, Caprioli RM, Mirnics K (2013) Mol Psychiatry
    › Citation · 24322205 (PubMed)
  4. Generation of a Tenascin-C-CreER2 Knockin Mouse Line for Conditional DNA Recombination in Renal Medullary Interstitial Cells. He W, Xie Q, Wang Y, Chen J, Zhao M, Davis LS, Breyer MD, Gu G, Hao CM (2013) PLoS One 8(11): e79839
    › Citation · 24244568 (PubMed) · PMC3823583 (PubMed Central)
  5. Claudin-1 regulates intestinal epithelial homeostasis through the modulation of Notch-signalling. Pope JL, Bhat AA, Sharma A, Ahmad R, Krishnan M, Washington MK, Beauchamp RD, Singh AB, Dhawan P (2014) Gut 63(4): 622-34
    › Citation · 23766441 (PubMed)
  6. B lymphocyte "original sin" in the bone marrow enhances islet autoreactivity in type 1 diabetes-prone nonobese diabetic mice. Henry-Bonami RA, Williams JM, Rachakonda AB, Karamali M, Kendall PL, Thomas JW (2013) J Immunol 190(12): 5992-6003
    › Citation · 23677466 (PubMed) · PMC3679359 (PubMed Central)
  7. Conditional loss of ErbB3 delays mammary gland hyperplasia induced by mutant PIK3CA without affecting mammary tumor latency, gene expression, or signaling. Young CD, Pfefferle AD, Owens P, Kuba MG, Rexer BN, Balko JM, Sánchez V, Cheng H, Perou CM, Zhao JJ, Cook RS, Arteaga CL (2013) Cancer Res 73(13): 4075-85
    › Citation · 23633485 (PubMed) · PMC3702683 (PubMed Central)
  8. The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation. Kartikasari AE, Zhou JX, Kanji MS, Chan DN, Sinha A, Grapin-Botton A, Magnuson MA, Lowry WE, Bhushan A (2013) EMBO J 32(10): 1393-408
    › Citation · 23584530 (PubMed) · PMC3655467 (PubMed Central)
  9. LRIG1 is a triple threat: ERBB negative regulator, intestinal stem cell marker and tumour suppressor. Wang Y, Poulin EJ, Coffey RJ (2013) Br J Cancer 108(9): 1765-70
    › Citation · 23558895 (PubMed) · PMC3658528 (PubMed Central)
  10. Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia. Ray KC, Moss ME, Franklin JL, Weaver CJ, Higginbotham J, Song Y, Revetta FL, Blaine SA, Bridges LR, Guess KE, Coffey RJ, Crawford HC, Washington MK, Means AL (2014) Oncogene 33(7): 823-31
    › Citation · 23376846 (PubMed) · PMC3929321 (PubMed Central)

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

No contact person provided

Keywords

Mouse (10), Knock in (1), ES cells (5), rederivation (5), cryopreservation (6), BAC (14), RMCE (9), Transgenic (3), Knockout (5), iPSC cells (1)