Transgenic Mouse / ESC Shared Resource

Transgenic Mouse / ESC Shared Resource

The mission of the Vanderbilt Transgenic Mouse/Embryonic Stem Cell Shared Resource (TMESCSR) is to provide services that facilitate the generation, storage and regeneration of genetically altered mice. We provide a wide array of services that are essential for the derivation, maintenance and long-term storage of genetically altered mouse models.  

The following procedures are provided on a fee-for-service basis:

  • Gene targeting in mouse ES cells
  • Pronuclear DNA microinjections
  • Recombinase Mediated Cassette Exchange
  • Embryo and sperm cryopreservation
  • In vitro fertilization and rederivation
  • BAC recombineering
  • Southern Blot Hybridization

The TMESCSR is staffed by experienced stem cell scientists and highly competent staff, and our track record in performing these services is extensive.  In addition, we are also happy to work with you to develop and/or implement new methods and techniques.  

  • Location: 9410 MRB-IV
  • Phone: 615-936-3454  

Publications/Citations

Featured publications

  1. mVps34 Deletion in Podocytes Causes Glomerulosclerosis by Disrupting Intracellular Vesicle Trafficking. Chen J, Chen MX, Fogo AB, Harris RC, Chen JK (2013) J Am Soc Nephrol
    › Citation · 23291473 (PubMed) · Added on 01/25/2013
  2. Ghrelin expression in the mouse pancreas defines a unique multipotent progenitor population. Arnes L, Hill JT, Gross S, Magnuson MA, Sussel L (2012) PLoS One 7(12): e52026
    › Citation · 23251675 (PubMed) · PMC3520898 (PubMed Central) · Added on 01/14/2013
  3. CSF-1 signaling mediates recovery from acute kidney injury. Zhang MZ, Yao B, Yang S, Jiang L, Wang S, Fan X, Yin H, Wong K, Miyazawa T, Chen J, Chang I, Singh A, Harris RC (2012) J Clin Invest 122(12): 4519-32
    › Citation · 23143303 (PubMed) · PMC3533529 (PubMed Central) · Added on 01/25/2013
  4. Dual lineage-specific expression of Sox17 during mouse embryogenesis. Choi E, Kraus MR, Lemaire LA, Yoshimoto M, Vemula S, Potter LA, Manduchi E, Stoeckert CJ, Grapin-Botton A, Magnuson MA (2012) Stem Cells 30(10): 2297-308
    › Citation · 22865702 (PubMed) · PMC3448801 (PubMed Central) · Added on 01/14/2013
  5. Differential regulation of embryonic and adult β cell replication. Gunasekaran U, Hudgens CW, Wright BT, Maulis MF, Gannon M (2012) Cell Cycle 11(13): 2431-42
    › Citation · 22659844 (PubMed) · PMC3404874 (PubMed Central) · Added on 01/29/2013
  6. Lineage-specific adjacent IFNG and IL26 genes share a common distal enhancer element. Collins PL, Henderson MA, Aune TM (2012) Genes Immun 13(6): 481-8
    › Citation · 22622197 (PubMed) · Added on 01/29/2013
  7. Generation of a conditional allele for the mouse endothelial nitric oxide synthase gene. Jiang R, Wang S, Takahashi K, Fujita H, Fruci CR, Breyer MD, Harris RC, Takahashi T (2012) Genesis 50(9): 685-92
    › Citation · 22467476 (PubMed) · PMC3399989 (PubMed Central) · Added on 01/25/2013
  8. Nkx2.2 repressor complex regulates islet β-cell specification and prevents β-to-α-cell reprogramming. Papizan JB, Singer RA, Tschen SI, Dhawan S, Friel JM, Hipkens SB, Magnuson MA, Bhushan A, Sussel L (2011) Genes Dev 25(21): 2291-305
    › Citation · 22056672 (PubMed) · PMC3219233 (PubMed Central) · Added on 01/14/2013
  9. A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1. Potter LA, Choi E, Hipkens SB, Wright CV, Magnuson MA (2012) Genesis 50(4): 384-92
    › Citation · 21913313 (PubMed) · Added on 01/29/2013
  10. Goodpasture antigen-binding protein (GPBP) directs myofibril formation: identification of intracellular downstream effector 130-kDa GPBP-interacting protein (GIP130). Revert-Ros F, López-Pascual E, Granero-Moltó F, Macías J, Breyer R, Zent R, Hudson BG, Saadeddin A, Revert F, Blasco R, Navarro C, Burks D, Saus J (2011) J Biol Chem 286(40): 35030-43
    › Citation · 21832087 (PubMed) · PMC3186396 (PubMed Central) · Added on 01/25/2013

Community Leaders

Address

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Keywords

Mouse (3), Knock in (1), ES cells (4), rederivation (4), cryopreservation (6), BAC (15), RMCE (9), Transgenic (1), Knockout (1), iPSC cells (1)